HPLC Column, Diamond Hydride, 4 um, 3.0 mm ID x 250 mm Length, 100 A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 70000‑25P‑3 Diamond Hydride HPLC Column is a high‑resolution solvent‑saving HILIC column, designed for laboratories that require maximum separation efficiency for polar compounds while significantly reducing mobile‑phase consumption and operating costs.
Why Choose a 3.0 mm × 250 mm Solvent‑Saver Column
This column combines two powerful advantages:
3.0 mm internal diameter for solvent reduction
250 mm column length for maximum chromatographic resolution
Compared to a traditional 4.6 mm ID column of the same length, this solvent‑saver format:
Reduces mobile‑phase consumption by approximately 50–60%
Operates at lower optimal flow rates
Produces lower back pressure at equivalent linear velocity
Maintains analytical‑scale robustness and injection tolerance
Unlike narrow‑bore LC‑MS columns, the 3.0 mm ID format is compatible with most conventional HPLC systems, allowing laboratories to achieve meaningful solvent savings without changing pumps, fittings, or detectors.
Maximum Resolution with 250 mm Length
The 250 mm column length provides a substantial increase in theoretical plates and peak capacity compared to 100 mm and 150 mm formats, making this column especially effective for:
Very complex mixtures of polar analytes
Closely eluting metabolites or structurally related compounds
Methods where shorter columns do not provide sufficient resolution
Research‑grade and regulated workflows requiring confident peak separation
This configuration is commonly selected when resolution is the primary analytical objective, but solvent usage and operational cost must still be controlled.
Diamond Hydride™ Stationary Phase Advantages
Built on Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase offers important performance benefits over conventional bonded‑silica HILIC phases:
Faster equilibration, even at high organic content
Stable and reproducible retention mechanisms
Extended column lifetime for routine and long‑term use
This chemistry is broadly adopted for:
Polar and non‑polar peptides
Amino acids and organic acids
Metabolites and other small biomolecules
Highly polar compounds problematic on traditional HILIC phases
Efficient, Economical High‑Resolution Operation
By pairing extended column length with a reduced internal diameter, the 70000‑25P‑3 allows laboratories to maximize resolution while minimizing solvent cost and waste generation. It is an ideal choice for groups that need analytical‑scale performance with improved sustainability and cost control, without transitioning to narrow‑bore or UHPLC‑specific hardware.
System Requirements
Typical operating flow rates: ~0.4–0.6 mL/min
Reduces mobile‑phase consumption by approximately 50–60% compared to 4.6 mm ID columns
Compatible with most conventional analytical HPLC systems
No special low‑flow or UHPLC‑specific pump requirements
Final flow optimization may depend on column length, mobile‑phase viscosity, temperature, and system pressure limits.